How to measure the photosynthesis of Arabidopsis leaves with a photosynthesis analyzer?
     
In the past few years of business consulting, customers have been inquiring about how to use the gas exchange method to determine the photosynthesis parameters of Arabidopsis leaves.
For this problem, there is no essential difference in the measurement of Arabidopsis leaves (or similar small leaf samples) from other plant leaves. The key difficulty is how to solve the problem of too small leaves in Arabidopsis. The problem that the blade is too small is: 1 only one small blade is measured at a time, because the area is too small (less than 1cm 2 ), the error is inevitably increased; 2 if multiple blades are measured at one time, how to accurately calculate the blade area of ​​the sample, Otherwise it will be a source of error that cannot be ignored.
   
Therefore, there are two problems to be solved for the photosynthetic assay of Arabidopsis leaves:
1 How to maximize the area of ​​each sample measured. The larger the measurement area of ​​the blade, the smaller the measurement error.
2 How to obtain the area of ​​the measured blade more accurately (Note: There is no photosynthetic instrument on the market with the function of automatically measuring the leaf area)
   
For the above questions, we recommend that you refer to the "Synthesis of Arabidopsis thaliana and in vitro leaf photosynthesis" (Zeng Xiaomei et al., Plant Physiology Newsletter, Vol. 38, No. 1, February 2002). The experiment in this article was completed by the academician Shen Yungang of the Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. It describes in detail the disposal methods and precautions of the blade, which is of great significance.
There is no mention in the literature how to obtain the area of ​​the blade. It is estimated that it is manually measured by the lattice method or the specific gravity method after the photosynthetic measurement is completed, and the data given by the instrument is corrected.
In addition, some customers asked about the effect of the "Israeli Leaf Chamber" on the market. Since no "Islandic Arabidopsis Leaf Chamber" on the market can automatically acquire the area of ​​the blade, it is impossible to fundamentally solve the above problems, and their prices are not cheap. In fact, the methods mentioned in the literature can be replicated in every laboratory.

Methenamine CAS No.100-97-0

Hexamethylenetetramine Basic Information
CAS: 100-97-0
MF: C6H12N4
MW: 140.19
EINECS: 202-905-8
Mol File: 100-97-0.mol

Hexamethylenetetramine Structure

Methenamine

Hexamethylenetetramine Chemical Properties
Melting point 280 °C (subl.)(lit.)
Boiling point 246.7°C (rough estimate)
density 1.33
refractive index 1.4260 (estimate)
storage temp. Store at RT.
solubility H2O: 1 M at 20 °C, clear, colorless
form Solid
Water Solubility 895 g/L (20 ºC)
Stability: Stable. Incompatible with strong acids, strong oxidizing agents.

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