The use of antibiotic residues in honey, the rapid test method of antibiotics is one of the concerns of all countries in the world. There are many methods for rapid detection of antibiotic residues in fresh milk in China, including: paper method, TTC method, enzyme-linked immunosorbent assay, etc. The method of using CSF-E96K antibiotic residue detector to detect fresh milk antibiotic residue adopts the principle of solid phase enzyme-linked immunosorbent ELISA, ie, enzyme-linked immunosorbent assay, which has the advantages of short time, simple operation and accuracy. Conducive to the strict quality control of agricultural raw materials. In this paper, the CSY-E96K antibiotic residue detector was used to detect the rapid test method for antibiotic residues in bee products. The results show that the method is feasible and suitable for the detection of antibiotics in honey and its products. . Below we specifically explain the detection steps of penicillin: Material 1. instrument CSF-E96K antibiotic residue detector, pulverizer, measuring cylinder, oscillator, funnel, filter paper, micropipette, deionized water or distilled water, methanol. 2. Reagent Penicillin kit experiment method 1 Take 10ml sample and add 20ml 70% methanol solution 2 strong oscillation for 3 minutes 3 Filter with Whatman No 1 filter paper 4 Take 100 μl of the treated sample and add 400 μl of the sample diluent. 5 Take 50 μl of the diluted sample for analysis (dilution factor 5) Results and discussion Pre-numbered, mark the position of B0, standard and sample, recommended for double hole detection 2 Take the required number of micro-holes (the micro-hole strips are detachable), re-seal the excess slats and immediately return them to 2~8 °C for storage. 3 Dilute the sample diluent (10×) and concentrated washing solution (20×) into working solution (diluted with distilled or deionized water) 4 Add 50μl of 0.0 ppb standard solution to B0 well 5 Add 50 μl of standard solution to each standard well. 6 Add 50μl sample solution to each sample well 7 Add 50 μl of Benzyl penicillin (BP) abzyme conjugate to all wells 8 Gently shake the plate for a few seconds. 2, 37 ° C warm bath 30min (during the temperature of the bath gently from time to time, can reduce the double hole error) 2.1 Remove the liquid from the well and wash the microplate 5 times with the wash solution. The last time should be tapped on the absorbent paper to completely remove the liquid from the well. 3 reaction 3.1 After the washing procedure is completed, immediately add 50 μl of coloring solution A to each microwell with a micropipette, and add 50 μl of coloring solution B; shake the reaction plate slightly to mix thoroughly. 3.2 37 ° C warm bath 10min 3.3 Add 50 μl of Stop Solution to each well and mix 3.4 Then place the instrument for testing, and the instrument automatically calculates the residue in 1 minute. Http:// Digital Safe,Digital Safe Box,Small Digital Safe,Electronic Digital Safe Ningbo Reliance Security Technology CO.,Ltd , https://www.reliancesafes.com