What animal is the ABGENT antibody prepared from? Most of our monoclonal antibodies were prepared by BALB/c mice, and polyclonal antibodies were mostly prepared by New Zealand rabbits. The customer antibody customization service also mainly uses these two animals. How do you distinguish specific target bands in the Western Blot test? The results of Western Blot detection of polyclonal antibodies usually show multiple bands. We compare the results of post-immune serum and pre-immune serum: both in the antibody and in the serum after immunization, but not in the pre-immune serum. The band of predicted molecular weight can be considered as a specific target band. Why is there no positive result for the Western Blot test I bought? There are some details in ABGENT's Western Blot experimental procedure that are key to avoiding background depth and no results. 1 Do not over-clean the membrane. Use a TBS containing 0.1% Tween-20 for 3 times, 5 minutes each time. Excessive cleaning may wash off the primary antibody. Proper cleaning conditions are good at eliminating background and getting the best results. 2 Do not close for a long time. Excessive closure can cause the signal to weaken. 3 Please confirm whether the antigen you are testing is the antigenic species listed in the product manual. How can we ensure the repeatability of the Western Blot experiment? There are many factors that cause poor repeatability of the Wester Blot experiment, which can be considered from the following aspects: 1 Whether the preservation conditions of the antibody are strict; 2 After the antibody is diluted, the working concentration is very low and cannot be used repeatedly; 3 Does the test sample contain the target protein? The amount of target protein in the cell lysate will be due to the number of cells Hotel Safe,Hotel Safe Deposit Box,Electronic Box Hotel Safe,Electronic Password Safe Deposit Box Hebei Hupai Aodi Cabinet Industry Co.,Ltd. , https://www.hupaiaodi.com