Raspberry is a perennial shrub of the genus Rubus. It is a cold temperate and small berry with strong adaptability. It is simple and easy to cultivate and easy to manage. The results of the second year of planting can be achieved. The third year will enter into the full fruit period. After that, it can yield and stabilize production for 15 to 20 years. The general variety yields around 15 000 kg/h. Raspberry contains multiple vitamins, of which vitamin C is 5 times that of apple and 6 times that of grape. Vitamin E content is the first of fruits, SOD, aminobutyric acid and other anti-aging, anti-cancer anti-cancer substances rich in content, roots and leaves also have medicinal effects. Due to the high nutritional value of raspberry and the quick-frozen export market, it has become an emerging fruit tree that is being vigorously developed and cultivated. It is called the “third generation fruit”. Raspberry berries are mainly used for processing in the international market, and are processed into juice, jam, fruit wine, jelly, chewing gum and various food additives, coloring agents, etc., which are used for juice production and wine production in sporadic cultivation in China, and are also used in Fresh food is a kind of natural health care fruit and medicine resources to be developed. Raspberry, Red Raspberry and Blackberry 2 series are the best in the 3rd generation of fruit popular in the world today. Due to its anti-aging substances such as vitamin E, SOD and aminobutyric acid, it is higher than existing fruits and other wild fruits. In particular, the anti-cancer substances of red raspberry are rich in osmic acid, and Europe and the United States call it "Ruby." The trial focused on a series of issues such as primary culture, subculture, rooting, and hardening and transplanting of black raspberry 'Triple Crown' and red raspberry 'Red Raspberry 38' selected in the United States. A systematic study was conducted to explore the optimum medium types, hardening and transplanting methods for blackberry 'Triple-crown' and 'Raspberry 38' tissue culture, laying the foundation for accelerating the large-scale industrial production of high quality raspberry seedlings. Provides technical basis for factory production of test tubes.

1 The author of the test material was from the Raspberry Professional Cooperative in Qingdui Village, Liuguang Township, Fengqiu County, Xinxiang City, Henan Province - the planted raspberry variety "Blackberry Triple Crown" and the "Red Raspberry" Red Raspberry 38 in the raspberry planting base. On the parent line of the ''strain'', strong shoots were grown as explants. These two varieties are suitable for cultivation in Central China, North China, and Northwest China, and their materials are quoted from the China Forestry Science Research Institute.

2 Explants on the basis We selected healthy and disease-free parent plants from the raspberry planting base in Qingdui Village, Liuguang Township, Fengqiu County, Xinxiang City, Henan Province, on July 16, 2008, and cut stem segments with strong growth potential. The stem tip is removed from the big leaf and put into a plastic bag to bring it back into the room for moisturizing.

3 Medium Preparation MS was used as the basic medium throughout the culture. 5〜4. 5 g/ L固化,分装后 After the different development stages of explants were added different types and concentrations of plant hormones, sucrose 3.0%, pH 5. 6 ~ 5. 8, agar powder 4. 0 ~ 4. 5 g / L curing, after packing Sterilized at 121 °C for 20 min, set aside. Among them: (1) Axillary bud-inducing medium MS + 6- BA 1. 0 mg / L + IBA 0.1 mg / L; (2) Subculture medium MS + 6- BA 1. 5 mg / L + IBA 0 03 mg/L; (3) Rooting medium 1/2 MS + IBA 0. 2 mg/L + activated carbon 0.2%.

4. Establishment of sterile lines The stem and stem tips of the removed leaves were washed under tap water for about 30 minutes to remove surface contaminants. The thorns and villi on the stem segments were removed with a razor blade and placed in a large beaker. , first soaked with 75% alcohol for 15 ~ 20 s, and then 0.1% liters of mercury solution for 15 min, and finally rinsed with sterile water 5 times, sterile filter paper to dry the water. Place the disinfected explants in sterile bottles for later use. This stage mainly solves the problems of sterilization and pollution prevention, and can improve the sterilization effect through strict operation of each link and the treatment of the mother plant with a bactericide before reprocessing.

5 Induction of axillary bud growth To disinfect the explants of raspberry, remove the contact between the two incisions and the disinfectant, cut it into small pieces with one axillary bud, and inoculate the axillary bud induction medium MS + 6- BA 1 0 mg / L + IBA 0.1 mg / L on culture. Room temperature (26 ± 2) °C, light intensity 1000 ~ 2000 lx, light 12 hours a day. After 5 d, the axillary buds started to grow. After 15 days of culture, the explants were all germinated, expanded and elongated, and the sprouts grew rapidly at 1.5 to 2.0 cm. A small amount of callus was generated at the base. . At the time of 20-25 days, when the shoots were 4-5 cm in length, the stem segments with 1-2 shoots were cut and transferred to fresh shoot induction medium. Ten days later, young shoots grew, a small amount of callus was formed at the base, and clustered shoots began to appear. Each of the explants ranged from 2 to 7 and 20 days later, the shoots were 4 cm long.

6 After subculture, the axillary buds of the growing seedlings were cut and inoculated on the subculture medium MS+6-BA 1.5 mg/L+IBA 0. 03 mg/L. After 30 days, shoots clustered were usually grown. The subculture period is 35 to 50 days, and the multiplication factor is 5 to 7 times. To obtain the high efficiency of tube propagation, the ratio of plant hormones is an important factor. In order to determine the preferred first author profile: Guo Yuxia (1980-), female, assistant researcher; engaged in the ratio of fruit trees and hormones, we configured to use MS as the basic medium, plus several different hormone concentrations of plant hormones Culture medium. During the experiment, it was found that the greater the ratio of 6-BA/IBA, the better the bud differentiation. The results showed that although the differentiation of the induction medium used in the experiment was less, the differentiated shoots were all effective shoots. The medium was finally selected as the best subculture medium. In addition, it was also found that when the subcultured inoculation operation was performed, cutting off the apical shoots of the shoots and then transferring them to the fresh medium had a great effect on the proliferation of shoots. Different raspberry varieties have very different growth at the same hormone level and under the same conditions. Therefore, in the use of raspberry for industrialized tissue culture growth, it is not necessary to blindly pursue an excessively high differentiation factor, and the optimum number of seedlings is required. Therefore, the concentration of 6-BA should not exceed 1.5 mg/L. The use of raspberry tissue culture technology for rapid propagation and robust seedling growth is the main route for propagation of seedlings of high quality raspberry varieties.

7 After inducing rooting and subculture to obtain more clustered shoots, shoots with a length of about 4 to 5 cm were picked and inoculated on the medium 1/2 MS + IBA 0.2 mg/L + activated carbon 0.02% to induce rooting. to cultivate. The cultivation conditions are the same as before. In this period, the selection of rootless cultures that are consistent and consistent with rootless seedlings will be crucial for the subsequent formation of standardized and standardized commercial crops in batches. About 5 days after inoculation, 3 or 4 robust white roots can be seen in the blackberry variety 'Triple Crown', while the 'Raspberry 38' requires about 10 days, and the shoots grow rapidly with 4 to 6 leaves. . Adding 0.2 g/L activated charcoal to the rooting medium had a good promoting effect on the rooting of some varieties of raspberry.

8 Transplanting the seedlings in the process of tissue culture, the success of the transplanting of the test-tube seedlings is also a key link, because no matter how well the test-tube seedlings grow in the test tube, if the transplanting is not successful, the previous work will be abandoned. In the test, when the test tube seedlings have 4 to 6 leaves and 5 to 6 roots, they can be transplanted. Before the transplanting, the test tube seedlings shall be ventilated and smelted. The rooted test tube seedlings shall be taken out of the culturing room first, and the sealing film shall be removed so that the tender seedlings will gradually come into contact with the outside world and enhance the adaptability. After hardening the seedlings for 3 days at room temperature, they are then transferred into bottles. Add a small amount of warm water to soften the medium. When transplanting, the test tube seedlings were gently removed with forceps, the root culture medium was rinsed clean, and transplanted into a mixed matrix of disinfected pure sand: peat moss = 1:1. In the treatment of the matrix, use mancozeb 1 000 times spray, thoroughly mix thoroughly and then drenched with water, pay attention to proper shading and moisturizing within 10 days after transplanting, and then open the shade, in between Spray water in a timely manner to ensure that the substrate is wet. The test results showed that the survival rate of transplanting plants was more than 90.0%.

Organic Chaga Extract

Organic Chaga extract powder is made from perennial Chaga fruit bodies. Chaga is an irregularly shaped fungus that usually grows on birch, eucalyptus, and beech trees. The fruit body of Chaga is composed of oxalic acid, formic acid, acetic acid, polysaccharides, fiber, and resin, which can improve appetite and eliminate pain. It can reduce the cholesterol content in the blood. According to academic research, in the 16th and 17th centuries, Chaga was widely used in Eastern Europe, Russia, Poland, Finland and other folks to prevent and treat various incurable diseases such as various digestive organ cancer diseases (stomach cancer, esophagus) Cancer, bowel cancer, liver cancer), heart disease, leukemia, diabetes, etc., have shown excellent results. Clinical trials from Russia and Japan have confirmed that Chaga significantly improves the body`s immunity, inhibits the proliferation and metastasis of tumor cells, and can effectively prevent cancer in patients with gastric ulcers.

Chaga Extract,Organic Chaga Extract Powder,Organic Chaga Extract,Organic Chaga Mushroom Powder

Organicway (xi'an) Food Ingredients Inc. , https://www.organicwayinc.com