Human (Human) Fibroblast Growth Factor 21 (FGF-21) ELISA Kit Instructions

This kit can only be used for scientific research and should not be used for medical diagnosis.

Al (Human) fibroblast growth factor 21 (FGF-21)

ELISA test kit

user's Guide

Detection principle

The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with fibroblast growth factor 21 (FGF-21) antibody, specimens, standards, and HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with fibroblast growth factor 21 (FGF-21) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration.

Sample collection, processing and storage methods

1. Serum: Use a tube containing no pyrogen and endotoxin. Avoid any cell irritation during the procedure. After collecting the blood, centrifuge and centrifuge for 10 minutes at 3000 rpm to quickly and carefully separate the serum and red blood cells.

2. Plasma: EDTA, citrate or heparin anticoagulation. The supernatant was taken by centrifugation at 3000 rpm for 30 minutes.

3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymer.

4. Tissue homogenization: The tissue is mashed by adding appropriate amount of physiological saline. The supernatant was taken by centrifugation at 3000 rpm for 10 minutes.

5. Storage: If the sample is not detected in time after collection, please dispense it once, freeze it at -20 °C, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is fully thawed evenly.

Bring your own items

• Microplate reader (450nm)
• High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
• 37 ° C incubator

Operational precautions

1. The kit was stored at 2-8 ° C and equilibrated for 20 minutes at room temperature before use. The concentrated washing liquid taken out from the refrigerator will crystallize, which is a normal phenomenon, and the water bath is heated to completely dissolve the crystals before use.
2. The slats not used in the experiment should be immediately put back into the ziplock bag and sealed (low temperature dry) for storage.
3. The S0 standard with a concentration of 0 can be regarded as a negative control or blank; the sample has been diluted 5 times according to the instructions, and the final result multiplied by 5 is the actual concentration of the sample.
4. Incubation is carried out in strict accordance with the time indicated in the instructions, the amount of liquid added and the order.
5. Shake well all liquid components before use.

Kit composition

Note: The concentration of standard (S0-S5) is: 0, 15, 30, 60, 120, 240 pg/mL

Reagent preparation

Dilution of 20× Wash Buffer: Distilled water was diluted 1:20, ie 1 part of 20× Wash Buffer plus 19 parts of distilled water.

Washing method

1. Manually wash the plate: Drain the liquid in the hole, fill each hole with the washing liquid, let stand for 1 min, then drain the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times.
2. Automatic washing machine: Inject 350 μL of washing solution into each well, soak for 1 min, and wash the plate 5 times.

Steps

1. The required slats were taken out from the aluminum foil pouch after equilibrating for 20 min at room temperature, and the remaining slats were sealed back to 4 ° C with a ziplock bag.
2. Set standard and sample wells, standard wells with different concentrations of standard 50μL;
3. The sample well was first added with 10 μL of the sample to be tested, and then the sample dilution was 40 μL; the blank well was not added.
4. In addition to the blank wells, 100 μL of horseradish peroxidase (HRP)-labeled detection antibody was added to each well of the standard well and the sample well, and the reaction well was sealed with a sealing plate, and incubated at 37 ° C in a water bath or incubator for 60 min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. 50 μL of each of the substrates A and B was added to each well, and incubated at 37 ° C for 15 min in the dark.
7. 50 μL of the stop solution was added to each well, and the OD value of each well was measured at a wavelength of 450 nm within 15 min.

Result judgment

Draw a standard curve: In the Excel worksheet, the standard concentration is used as the abscissa, and the OD value is plotted as the ordinate. The linear regression curve of the standard is drawn, and the concentration values ​​of each sample are calculated according to the curve equation.

Kit performance

• Accuracy: The R value of the linear regression of the standard and the expected concentration is greater than or equal to 0.9500.
• Sensitivity: Detection of 1-240pg/ml, the minimum detection concentration is less than 1.0 pg/mL.
• Specificity: Does not cross-react with other soluble structural analogs.
• Repeatability: The coefficient of variation between the plates and the plates is less than 15%.
• Storage: 2-8 ° C, protected from light and moisture.
• Validity: 6 months

Disclaimer

• The kit is for research use only and should not be used for clinical trials or human experiments. Otherwise, the consequences will be borne by the experimenter and the company will not be responsible.
• In strict accordance with the instructions, the experimenter violates the instructions, and the consequences are borne by the experimenter.

Sour Gummy Candy

Introduction:
Are you a fan of the perfect combination of sweet and sour flavors? Look no further than Sour Gummy Candy – the ultimate treat for those seeking a tongue-tingling experience. Bursting with fruity goodness and a delightful tang, these candies have become a popular choice for both kids and adults alike. In this article, we will explore the irresistible charm of Sour Gummy Candy and why it deserves a spot in your snack collection.
1. A Flavor Explosion:
Sour Gummy Candy is renowned for its intense flavor profile that awakens your taste buds. With a delightful blend of mouth-puckering sourness and fruity sweetness, these candies offer an explosion of flavors with every bite. From zesty lemon and tangy lime to juicy strawberry and refreshing watermelon, each gummy candy is a burst of vibrant taste that will leave you craving for more.
2. Versatile Snacking Option:
Whether you're looking for a quick pick-me-up during the day or a treat to enjoy during movie nights, Sour Gummy Candy fits the bill perfectly. These candies come in various shapes and sizes, making them ideal for snacking on the go or sharing with friends and family. Their chewy texture adds an enjoyable element to the experience, making them a delightful indulgence for all occasions.
3. A Fun and Nostalgic Experience:
Sour Gummy Candy brings back memories of childhood for many, evoking a sense of nostalgia and joy. The vibrant colors, playful shapes, and the thrill of the sourness create a fun and exciting experience that transcends age. Whether you're reliving your childhood or introducing the joy of Sour Gummy Candy to a new generation, these candies are sure to bring smiles and laughter.
4. A Treat for All Ages:
Unlike some candies that are primarily enjoyed by children, Sour Gummy Candy has a broad appeal that transcends age groups. From kids who love the sour kick to adults who appreciate the burst of flavors, these candies are loved by all. They make for a perfect addition to birthday parties, family gatherings, or even as a little treat for yourself after a long day.
5. Quality Ingredients and Variety:
Sour Gummy Candy is crafted with care, using high-quality ingredients that ensure a delicious and consistent taste. Many brands offer a wide range of flavors and options, allowing you to choose your favorites or explore new combinations. Whether you prefer classic flavors or unique twists, there's a Sour Gummy Candy out there to suit every palate.
Conclusion:
Sour Gummy Candy is more than just a sweet treat – it's an experience that tantalizes your taste buds with its tangy delight. With its intense flavors, versatility, and nostalgic appeal, these candies have become a beloved snack for people of all ages. So, why not indulge in the mouth-watering goodness of Sour Gummy Candy and add a burst of tangy joy to your snacking routine?

Sour Gummy Candy,Gummy Sharks Candy,Sour Gummy Worms Candy,Custom Sour Gummy Candy

Montreal Shantou Food Co., Ltd , https://www.montrealsnack.com