Pathogenic strains of E. coli infect a wide range of mammals and birds. Poultry is more common in chickens, turkeys and ducks. However, the colibacillosis of guinea fowl is also a frequently-occurring and common disease, which is one of the causes of the death of guinea fowl. In particular, rare bird farms with long construction time and poor disease prevention conditions are more common. Therefore, to improve the survival rate of guinea fowls must be the prevention and treatment of this disease. 1 Incidence and clinical conditions The guinea fowls and chicks reared on this farm are all zoned and there are breeder farms on the north side of the farm and 2km southwest. In July 2004, some chickens in the flock were diarrhea and accompanied by respiratory symptoms. The breeder had no effect after two days of convulsing Sterling and Virus, and similar symptoms appeared in the flock on the third day. See 8 chickens depressed, loss of appetite, can not afford lying, lying in the mouth to remove sticky substances, and some grayish white sticky feces, body temperature 40.5 ~ 41.8 °C, 4 of them died within 12h. One of the longest duration died after 36 hours. Affected chickens have symptoms such as difficulty breathing and swollen head. 2 necropsy The death of the guinea fowl was thickened, with a cheese-like exudate on the respiratory surface, a cloud-like pericardium, epicardial edema, and pale red exudates. The extracardiac sac is filled with egg yellow fibrin exudate. There are fibrinous exudates and free yolk in the abdominal cavity. Ocular lesions are characterized by total ocular inflammation, monophagic anterior chamber empyema, blindness. Most dead chickens have swollen heads and face asymmetry. Enteritis lesions were the most prominent, and the small blood vessels of the duodenal mucosa ruptured particularly prominently. The mucosa of the small intestine was thickened significantly, and the contents of the intestines were yellowish red. After scraping the contents, the intestinal wall showed a cord-like haemorrhage. Local intestinal hemorrhagic lesions, a large number of fresh bleeding points. The other bowel segments have different lesions. There is a lot of fibrinous exudates on the surface of the liver, exudates are easy to peel, there are no obvious lesions inside, some lung tissue congestion. The liver was slightly swollen, with scattered bleeding points, swelling of the kidneys and deposition of urate, and appearance of patchy appearance. 3 Laboratory inspection 3.1 Isolation and Cultivation of Bacteria Sterilely take blood and liver disease from 3 dead guinea fowls, first burn the tissue with a blade, and then remove the sample by burning the surface tissue with the inoculation loop and streak the culture on the common agar medium. At 37° C., colonies formed after 24 h. As a result, the three colonies were similar. The colonies were low and protuberant, smooth and colorless, and the edges of 1 to 3 mm in diameter were neat. The colonies were inoculated in a broth medium and grew well, producing turbidity. The lesions were streaked on EMB agar and MacConkey agar media. Most colonies on eosin blue were characteristically black and had metallic flashes on MacConkey agar. Bright red colonies appeared. 3.2 Bacterial biochemical tests The sugar fermentation experiments showed that the three strains can ferment glucose, maltose, and mannitol, and produce acid gas, but do not decompose dextrin, starch, and inositol. The methyl red test is positive, and the VP test is negative. The bacteria is E. coli. 3.3 Drug susceptibility testing The use of antibiotics in chemotherapy has been widely used in the treatment of E. coli, but it varies greatly. This depends to a large extent on whether the treatment is timely and what kind of drug is used. Due to the different sensitivity of different Pasteurella strains to chemical drugs, drug resistance sometimes appears. Therefore, it is very necessary to conduct drug sensitivity test. The drug susceptibility test was performed according to the conventional paper method. The experimental results show that the strain is highly sensitive to chloramphenicol, picoside and compound flavomycin; it is moderately sensitive to tetracycline and streptomycin; it is insensitive to penicillin, erythromycin, kanamycin and enemy bacteria. 4 Control measures 4.1 The compound flavomycin was added to the feed, and 5% of the enrofloxacin was added to the drinking water for 5 days. There was no incidence of healthy guinea fowl. In order to control the epidemic of the disease, we used a 0.25% poison spray to disinfect the ground and walls of the birdhouse. The virus was disinfected once a day for three consecutive times. The sports field and fence were disinfected with 2% fire alkali water, and the door was sterilized. Fresh lime. 4.2 Isolation therapy was performed on the affected chickens. In addition to feed and drinking water, the rats were intramuscularly injected with chloramphenicol 400,000 IU/kg once daily for 5 days. One week after the above-mentioned measures, the spirit of the flock has obviously improved and the epidemic has been effectively controlled.
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