The strict requirements of artificial insemination of breeder chickens can be summarized as follows: the specification of the action and the skill of the breeder. Since the male and female chickens reflect the specific conditions formed by sperm sampling and sperm insemination, the operation must be meticulous, the actions should be coordinated properly, and the municipality should be able to achieve a unity between the human and the chicken to ensure good sperm extraction and qualified insemination rates. . The following operating procedures are available for reference.

First, the choice of breeding roosters

Roosters for artificial insemination should be healthy, disease-free and in line with the requirements of chicken species. The crown is well developed, the abdomen is soft, the legs are stout, the massage can evert the anus, the device can erect, and the quantity and quality of semen are better. The male-female ratio is preferably 1:20-1:25, plus 5% of the reserve cock.

Second, the preparation of artificial insemination

1. personnel. Four seriously selected and well-qualified breeders were divided into two groups to perform sperm collection and insemination. Semen and insemination personnel should be relatively fixed.

2. instrument. All the above instruments were rinsed with clean water. After washing, they were boiled with boiled water for 15 minutes, rinsed with saline and soaked for 10 minutes, and dried after use.

Three, cock tuning and mining

1. Semen operation. Before harvesting, first trim the feathers around the anus of the rooster to avoid contamination. Before the formal use of cock, the cock must be trained to form a specific condition reflex. Normally 3-4 days of training, training and sperm collection must be completed by 2 people. One person grasps the chicken and collects the test tube, one person collects sperm and weighs the semen. Method: One person catches the chicken, the tail is forward, the head is back, is fixed in the armpit, the legs are natural stance, the extrude member clamps the set fine cup in the middle of the right hand and the ring finger, and the mouth is facing outward and the back of the hand, the left hand Separate the thumb from the four fingers and place it on the inner sides of the rooster's back. Swing the tail area and gently massage 2-3 times. At the same time, separate the right thumb and four fingers and gently massage the lower edge of the phalanges to treat the cock. When there is a strong sexual reflex (the evacuation of the mating device), immediately raise and squeeze with the index finger and thumb of the left hand (without retracting the mating device), and with the right hand, invert the cup of semen with the sperm-collection cup, and move immediately. Collect sperm in a test tube.

2. Precautions

(1) Roosters should be fasted 2 hours before picking to reduce pollution.

(2) Gather semen only from milky viscous semen. Yellow semen contains feces, brown semen with blood, urate in thin semen, not suitable for insemination.

(3) When collecting sperm, avoid water, alcohol and disinfectant as much as possible.

(4) Semen collection should be conducted 1 time every other day or 3 days off for 4 days.

IV. Insemination

1. Insemination operation. The entire process of insemination can be completed by one person. Method: One person catches the chicken. The left thumb is placed under the anus. The other four fingers are placed above the anus. Extruding forward with force, the cloacal ectropion can be seen, and the red side of the left side is the hatching mouth. At this time, the inseminator used the vas deferens with the insemination needle to inhale the semen 0.02-0.03ml into the 2-3cm of the infusion ovum to enter the vas deferens quickly, and at the same time, the anus was immediately let go.

2. Precautions

(1) Insemination should be performed after 2-3 pm, and water should be stopped 1-2 hours before insemination. (2) The insemination needle is changed once. (3) The newly collected semen is used within 30 minutes, otherwise it will affect sperm motility. (4) The insemination cycle is 5 days or 6 days, and the time interval is too long, which affects the fertilization effect.

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