About Waters Corporation () Portable Vet Ultrasound Series
Wireless Ultrasound for animal
XProbe-C Convex, suit for big placenta of cattle and horse
XProbe-L Linear, suit for small placenta of cattle and horse
XProbe-N Suit for sheep
Feature:
· High cost-effective
·Wireless connectivity, easy to operate
·Small and light,easy to carry
Specifications:
-Scanning mode: Electronic array
-Image mode: B, B/M
-Frequency: Convex 3.5MHz/5MHz, Linear 7.5MHz/10MHz
-Scan Depth: Convex 90/130/160/200mm, Linear 20/40/60/100mm
-Probe Radius/Length: Convex R60, Linear L64
-Image Adjust: Gain, DYN, Focus, Depth, Harmonic, Denoise
-Puncture assist function: the function of in-plane puncture guide line, out-of-plane puncture guide line, automatic blood vessel measurement, and the enhancement function of needle point development.
-Cineplay: auto and manual, frames can set as 100/200/500/1000
-Measure: Length, Area, Angle, Obstetrics
-Image frame rate: 18 frames/second
-Image save format: jpg, avi and DICOM
-Battery working time: 6-8 hours
-Length: 800mm
-Weight: 600g
-Wifi type: 802.11g/20MHz/5G/450Mbps
-Working system: Android
Portable Vet Ultrasound,Vet Untrasound Probe,Veterinary Ultrasound Machine Portable,Animal Ultrasounder Scanner Guangzhou Sonostar Technologies Co., Limited , https://www.sonoeye.com
For more than 50 years, Waters Corporation (NYSE: WAT) has made significant advances in medical services, environmental management, food safety and global water quality monitoring by providing practical and sustainable innovations that have created business for laboratory-related organizations. Advantage.
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Waters ACQUITY UPLC H-CLASS-PDA System and ACQUITY UPLC/Xevo TQ MS System
Analysis of 2-methylimidazole and 4-methylimidazole in beverages
Zhao Jiayu. Cai Wei. Sun Qinglong
introduction
Caramel coloring is a coloring agent that is allowed to be used. In addition to the individual products, the coloring amount of the caramel color is used in an appropriate amount according to the production requirements, and it is stipulated that only the caramel color produced by the ammonium sulfite method is allowed to be used in the carbonated beverage. Caramel (class III ammonia caramel and class IV ammonium sulfite caramel) made with ammonia or its ammonium salt will produce 4-methylimidazole, and 4-methylimidazole is a tumor that can induce tumors. High level of chemicals.
Caramel color is widely used in foods and beverages, so the monitoring of 4-methylimidazole content must also be taken seriously. Because of the high polarity and low content of 4-methylimidazole, how to detect it quickly and accurately Out of its content, it has become the focus of research at this stage. At present, there is only "the determination of 4-methylimidazole in caramel color - high performance liquid chromatography" in China's national standards, but there is no relevant detection method for 4-methylimidazole in beverages.
The total solution provided by Waters ® also monitors 4-methylimidazole and 2-methylimidazole in beverages. Use Waters SPE's solid phase extraction strategy to purify complex sample matrices, complete extraction and concentration of 4-methylimidazole and 2-methylimidazole, while Waters HILIC mode chromatographic retention for polar molecules The chromatographic separation provides perfect results, and finally through the analysis of UPLC ® H-CLASS PDA and UPLC / Xevo ® TQ MS, excellent qualitative and quantitative work.
Experimental condition
Sample preparation protocol
Solid phase extraction SPE solution - Oasis ® MCX (3cc / 60mg) cartridge purification 3g beverage samples, ultrasonic 5 minutes, after purification.
Activation/equilibrium
3mL methanol / 3mL water
Upper 3mL sample
Cleaning A: 2% aqueous formic acid / B: 100% methanol
Elution of 5% ammoniated methanol
Nitrogen
Acetonitrile: methanol (9:1) constant volume
On-machine analysis
ACQUITY UPLC H-CLASS PDA Ultra Performance Liquid Chromatography Separation Conditions:
Column: ACQUITY UPLC ® BEH HILIC Column; 2.1x100 mm, 1.7μm
Mobile phase A: acetonitrile
Mobile phase B: 5 mM ammonium formate
Column temperature: 35 ÌŠC
Detection wavelength: 215nm
Injection volume: 5μL
Running time: 3min
Gradient table: Time (min) Flow (mL/min) %A Curve
0.00 0.5 80 6
3.00 0.5 80 6
ACQUITY UPLC Xevo TQ MS Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry Analysis Conditions:
Column: ACQUITY UPLC BEH HILIC Column; 2.1x100 mm, 1.7μm
Mobile phase A: acetonitrile
Mobile phase B: 5 mM ammonium formate
Column temperature: 35 ÌŠC
Injection volume: 2μL
Running time: 3min
Gradient table: Time (min) Flow (mL/min) %A Curve
0.00 0.5 80 6
3.00 0.5 80 6
Experimental results and discussion
1. ACQUITY UPLC H-CLASS PDA Analysis
Mixed standard chromatogram
Beverage blank sample drawing
Matrix addition recovery chromatogram
2. ACQUITY UPLC/Xevo TQ MS Analysis
Mixed standard TIC
3.2.3 Analysis of spiked and blank comparison of tea beverage samples
3.2.4 Contrast analysis of cola sample spikes and blanks
It can be seen from the analysis results that the molecules of 4-methylimidazole and 2-methylimidazole are very polar, generally difficult to retain in reversed phase, and ion-pairing reagents are often used to increase retention, but due to the long balance time of ion-pair chromatography, Increasing the overall analysis cycle, while at the same time causing large losses on the column and instrument, the most critical is the inability to perform effective mass spectrometry analysis. The Waters HILIC mode polarity analysis program can very well carry out the retention of polar molecules, the mobile phase is simple, and it is compatible with mass spectrometry conditions, so that 4-methylimidazole and 2-methylimidazole have very good separation effect. And sensitivity.
At the same time, because the target compound is very polar, the requirements for pretreatment are very high, and separation and extraction is a difficult point, and Waters' solid phase extraction scheme can achieve a very good purification effect, and the separation is separated by Oasis MCX. It can reduce the damage of sample impurities to the column and the entire instrument system. The ultra-efficient performance and sensitivity provided by Waters ACQUITY UPLC H-CLASS-PDA and ACQUITY UPLC / Xevo TQ MS make the analysis of 4-methylimidazole and 2-methylimidazole ideal.
in conclusion
1. ACQUITY UPLC H-CLASS-PDA and ACQUITY UPLC / Xevo TQ MS can quickly and efficiently determine the content of 4-methylimidazole and 2-methylimidazole. The sensitivity of ACQUITY UPLC H-CLASS-PDA can reach 1mg/ Kg, ACQUITY UPLC / Xevo TQ MS sensitivity can reach 1μg / kg.
2. Apply Waters solid phase extraction SPE solution with HILIC mode chromatographic retention, which has good retention and separation and extraction for large polar small molecules, achieving ideal purification effect and chromatographic separation effect.
3. The overall solution from sample preparation to sample chromatography and mass spectrometry provides customers with integrated services to solve all problems that may be encountered during sample analysis and help customers succeed!