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Types of antibiotics
Thousands of antibiotics have been reported, which can be divided into six types according to their structure:
(1) sugar derivatives, mainly composed of hexosamine derivatives, such as streptomycin;
(2) polypeptide antibiotics, mainly or entirely composed of amino acids, have some characteristics of polypeptides or proteins, such as polymyxin, penicillin;
(3) polyene antibiotics, with multiple double bonds in molecular structure, such as nystatin and amphotericin;
(4) macrolide antibiotics, which are composed of one or more monosaccharides and together with carbon chains form a large aromatic lactone compound, such as erythromycin;
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1. Factors affecting plasma temperature are:
1 carrier gas flow rate: the flow rate increases, and the temperature of the central part decreases;
2 carrier gas pressure: the excitation temperature increases as the carrier gas pressure decreases;
3 Frequency and input power: The excitation temperature increases with the increase of power, which is approximately linear. When other conditions are the same, the frequency is increased and the discharge temperature is lowered.
4 Effect of the third element: The plasma of the release agent with a low ionization potential will increase the electron temperature.
The temperature of the plasma significantly affects the excitation intensity of the element to be tested, further affecting the detection limit of the element to be tested.
2. Calibration of sensitivity drift in analytical analysis of ICP spectrometer:
During the measurement process, the gas pressure change will affect the atomization efficiency and the distribution of the ground state atoms; in addition, capillary blockage, poor waste liquid discharge, will affect the solution lift and atomization efficiency; and voltage changes and even ambient temperature, etc. A number of factors can cause the sensitivity to drift. The calibration method can add a quality control sample close to the sample composition for every 10 samples measured, and appropriately shorten the standardized time interval according to the newness of the instrument used.
3. How to avoid mutual contamination between samples in ICP analysis?
When measuring, do not measure the samples with large concentration difference in sequence. The samples with similar concentrations can be measured together. Between the samples, rinse with distilled water. When entering the standard sample test standard curve, it should be from low to high. Injection.
4. In the ICP spectrometer analysis method, the acid used to decompose the sample must satisfy the conditions: as soon as possible, the various elements are rapidly and completely decomposed; the amount of the element to be tested is negligible; when the sample is decomposed, the element to be tested is not Should be lost; no insoluble matter is formed between the elements to be tested; the influence of coexisting elements is small when measuring; the atomizer and the torch are not damaged. Hydrofluoric acid can quickly damage the atomizer, the mist chamber and the torch.
5. Precautions for preparing multi-element stock standard solution for analysis of ICP spectrometer:
The solvent is made of high-purity acid or ultra-pure acid; using re-distilled ion-exchanged water; using spectrally pure, high-purity or reference material; some elemental elements are divided into several groups to avoid spectral interference or precipitation. For example, when measuring silicon, it is necessary to measure silicon separately.
6. In the ICP spectrometer analysis, why should we pay special attention to the configuration of the standard solution?
Incorrect configuration methods will lead to system deviation; media and acidity will not be suitable, and precipitation and turbidity will occur; improper grouping of elements will cause spectral interference between elements; reagents and solvents will not be pure enough, which will cause blank value increase and detection limit. The variation and the error increase.
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