Instructions for using the membrane for blood filtration (applicable to the company's recommended model GF2; whatman-MF1 and domestic MF-B02)
A: Affirmation, this description is the experience of the company's technical staff for many years of work, please reprint the person from Shanghai Jieyi Biotechnology Co., Ltd.; the description does not dare to say the best, if you find a better treatment during use, welcome Contact, exchange and learn from each other.
2: Before using the blood filter membrane, please understand that the purpose of using this consumable is to make the product more convenient and simple, so that the terminal user of the product does not need to centrifuge the blood into serum, which further saves the detection time and increases the efficiency. Use peripheral blood or fresh blood.
Three: When using the blood filter membrane, the packaging form of the final product should be taken into account. When facing the bare strip and the plastic card with the outer packaging, the blood filter membrane has different designs. A: bare strip, generally used The filter membrane on the bare strip should be considered for longer size, and the manufacturer of the general bare strip tends to produce a narrower product. (2.5MM-3.0MM) requires the length of the membrane to be designed. The length of the filter is sufficient to satisfy the sufficient blood filtration effect. Generally, the length of the filter membrane is selected from (17MM---30MM), which is inversely proportional to the width of the final product. The narrower the strip is used, the longer it is. The blood filter membrane, like the bare strip above, if it is 2.5mm wide, at least the filter membrane with a length of 20MM or more is used. In addition, the general bare strip has a MAX line label, it is best to add a layer of sealing tape at the intersection of the NC film, the gold pad and the blood filter film. (Recommended the company's adapter tape) Note that only tape is needed when pasting the tape. Cover to the NC film 1-2MM wide, the other parts are attached to the gold pad and the blood filter membrane, most of which are covered on the blood filter membrane, about 4-8MM (can be), such operation It can prevent the end customer from dropping the blood volume as required, and some even exceed the requirement of twice the specification. In this case, the sealing tape acts as a shut-off function, effectively preventing blood from filtering. The film directly runs on the NC film, and the sealing tape also plays a role in strengthening the connection of the NC film gold pad and the blood filter film in general terms; B: the product with the outer packaging plastic card, generally the narrowest of the products Will be less than 3.5MM, generally selected in 4MM, 5MM and 6MM, as mentioned above, the length of the blood filter membrane is inversely proportional to the width of the paper strip. Generally, the length of the filter membrane selected by 4MM and 5MM is 17MM or 20MM. The same principle as A, should choose the sealing tape to attach the NC film and the gold pad and the blood filter film, the principle is the same as the case A, but B because of the addition of a plastic packaging card, you can fully consider the filter when the card is opened. Blood matters, you can save the ring of sealing tape, close the card to the end of the blood filter film (by the gold pad end) 1/4, design a small card slot on the plastic card cover, let It forms a natural intercepting effect and must be designed to be loose in the area of ​​the sample well to meet viscous blood testing requirements.
Four: When attaching the blood filter membrane, please pay attention to one, that is, the uneven side of the blood filter membrane is facing upward, and the flat side is pressed down to the bottom plate, otherwise the effect is not good.
5: The preservation of the blood filter membrane is best preserved in a sealed dry environment. The ambient temperature is 2 - 37 degrees. If this condition is not met, the dry environment of the workshop (relative humidity below 40%) must be pre-released for half an hour before use. Then use it again.
Six: GF2; whatman-MF1; MF-B02 packaging specifications and prices are: 25CM * 30CM / Zhang (120 yuan / Zhang); 17MM * 50M / volume (926.90 yuan / volume); 21CM * 30CM / Zhang (50 yuan / Zhang); Arrangement of use effect: GF2 is best; whatman-MF1 is second; MF-B02 last ///// is calculated by the 4MM width of the terminal product, minus the loss, according to each large board (30CM large board) 70 Strip calculation, using GF2; whatman-MF1; MF-B02 corresponding production cost is: 0.137 yuan / root; 0.079 yuan / root; 0.068 yuan / root
GF2; whatman-MF1; MF-B02 respectively correspond to the use of: high-end products; mid-range; low-end products.
Seven: Take the 4MM wide strip of the terminal product as an example. Generally, the blood volume is controlled between 100ul and 150ul, and the natural drip tip blood is 4-5 drops. The final amount should be repeated and repeated gradient loading. After the experiment, 100% of the chromatographic detection process was successfully completed, and the minimum amount of the sample was added to 10%, and then the instruction amount was added.
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