2. Hidenori Tabata and Kazunori Nakajima. Chapter 14 In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neurons. "Electroporation And Sonoporation in Developmental Biology " http:// -2 Note: Mr. Hayakawa (Mr. Hayakawa), CEO and Chief Technical Designer of NEPA GENE, Japan, is the designer and manufacturer of CUY21. BEX is an OEM manufacturer that was commissioned by NEPA GENE to produce CUY21. Now NEPA GENE has replaced its OEM manufacturer and developed a new generation of NEPA21 versatile high-efficiency gene transfection system. For Mr. Hayakawa's contribution to electrical transfection technology, please refer to "Electroporation And Sonoporation in Developmental Biology" - the editor of the book and the pioneer of chicken embryo transfection, Mr Nakamura, clearly affirmed Mr. Hayakawa's contribution in the first chapter. Click to read the full text of the book ( http:// ).
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After nearly 30 years of development and innovation, electrical transfection has become an indispensable technical means in the field of functional research of genes. The following is not only an introduction to a newly-launched transfection instrument, but also an introduction to the technological innovation of the electrical transfection instrument because:
NEPA21 efficient gene transfection system    Â
------With the world's leading patented electric pulse chip technology and new electrical transfection program design
NEPA GENE specializes in the research and development, production of cell electro-transfection devices and electrofusion devices, etc. Its production of CUY21 series ( http:// ) electric transfection instrument has a long-standing reputation in the research field. It has been cited in hundreds of articles, including high-level magazine articles such as Nature, Cell, PNAS, Genes & Dvelopment.
NEPA GENE applied to the history of developmental biology research
NEPA GENE's products have a long history of use in the field of developmental biology.
In the early 1990s, when people began to use electroporation technology in developmental biology research, it was found that electric pulse caused great damage to animals, so subsequent research was difficult. Although the virus method can achieve better efficiency, there is a problem that the transfection site is not specific enough, and there is an immune reaction or other safety hazard.
Mr. Hayakawa (Mr. Hayakawa), the founder of NEPA GENE, was an agent of a well-known brand at the time, and was invited by Japanese laboratories to jointly innovate electroporation technology and instruments. After the success of technological improvement in 1994, Japan has reached the world's leading level in the technology and application of chicken embryo transfection. Mr. Hayakawa applied for a patent for the improved technology and equipment, and established NEPA GENE, and began to independently develop and produce electric transfection instruments.
"Electroporation And Sonoporation in Developmental Biology" is a professional book that introduces the application of electroporation technology in developmental biology. The book's editor-in-chief and the pioneer of chicken embryo transfection, Mr Nakamura, clearly affirmed Mr. Hayakawa's contribution in the first chapter. Click to read the full text of the book ( http:// ). The world's first scientist to report the transfection of mouse embryonic brain by electroporation, Dr H Tabata of Keio University, Keio University, was also completed by NEPA GENE (Refs. 1, 2). Please click to view the live transfection video demonstrated by Dr Tabata: http://video.sina.com.cn/v/b/63556016-2484835144.html
Newly designed electric transfection instrument NEPA21
In 2011, NEPA GENE launched a new generation of versatile NEPA21 efficient gene transfection system.
Conventional electric transducers use an exponentially decaying wave or a square wave waveform to electroporate the cell membrane. NEPA21 uses a patented three-step electro-transfection procedure to efficiently introduce foreign genes into cells while perforating the cell membrane. And with the unique reverse transfer (Reverse Transfer) and voltage decay (Voltage Decay) design, it can improve the cell viability while improving the transfection efficiency.
Can you achieve high transfection efficiency and high survival rate without special transfection kits ?
Transfection methods that are currently in common use require the help of chemical reagents, which typically cost $8, $12, or even $16 per sample. These auxiliary agents may have side effects that affect cell growth rate, apoptosis, or drug sensitivity. In addition, although electroporation transfection has been developed for nearly 30 years, no electrochemical transfection equipment is required for chemically transfected cells.
The situation has changed with the emergence of NEPA21. NEPA GENE's design philosophy is to develop the most sophisticated DNA and RNA transfection equipment and to change the traditional transfection methods that require special reagents. NEPA21's electrical pulse chips, programming and other technological innovations are world-leading. For example, it can produce high-precision low-voltage pulses, precise control of pulse time, and its capacitor design can also reverse electrode reverse discharge. For these reasons, NEPA21 enables efficient transfection of a wide variety of cells, tissues, and even living animals without the need for special reagents.
Application and innovation in in vitro (In Vitro ) / cell transfection - adherent transfection electrode
Cells can be transfected in suspension (In Suspension) or in Adherent state. Applicable to a variety of cells, including a variety of difficult to transfect cells, such as primary cells, stem cells, nerve cells, blood cells, immune cells.
When the cells are electrotransfected in suspension, they can be in 360 degree contact with the foreign gene, and the perforation efficiency and gene introduction efficiency are the best. NEPA21 has designed a variety of electric rotors to suit the transfection needs of different cell numbers. ( http://)
However, some primary cells are terminally differentiated, such as primary neuronal cells, neonatal rat cardiomyocytes, and the like. Once these cells are isolated from living tissues and cultured in an adherent manner, they cannot be digested and passaged, and transfection in a suspended state cannot be achieved. In response to this situation, NEPA GENE has developed an adherent transfection electrode for common cell culture plates - so that the experimenter can simply electrolyze the cells by simply plating the cells in a common 24-well or 6-well plate. Dyed!
In addition, NEPA21's patented electroporation program not only forms channels on the cell membrane, but also acts on the nuclear envelope to transport DNA directly into the nucleus. Therefore, even in terminally differentiated cells, DNA can enter the nucleus without relying on cell division.
Ex Vivo/ In vivo transfection - up to 250 electrodes, customizable
With up to 250 active transfection electrodes, NEPA21 covers almost all tissue, organ and animal transfection needs. For example, the muscle, skin, liver, kidney, testis, ovary, brain, retina, cornea and the like of rats or mice can also be used for transfecting animals such as fish and bees, as well as transfecting plant seeds and obtaining transgenic plants. These electrodes allow you to experiment with many previously difficult transfection experiments. If you have a new idea, NEPA GENE can also customize special electrodes to meet the transfection needs of specific sites based on the customer's genius. Click here to learn about the electrode selection method of NEPA21, and start the free living transfection test ( http:// )
Whether you need to transfect specific sites, such as the brain tissue of a mouse embryo, the cerebral cortex or stomach of a chicken embryo, or the need to transfect whole embryos, NEPA GENE can provide personalized advice, advice and services. .
references
1. Tabata H, Nakajima K. Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex. Neuroscience. 2001;103(4):865-72.