Tremella is a kind of expensive medicine and food dual-use bacteria, which has the effect of nourishing Shengjin, moistening the lungs and nourishing the stomach. China is a big country producing white fungus. The main production is concentrated in Tongjiang area of ​​Sichuan and Fujian. With the development of Tremella cultivation technology, the production of Tremella in China has been greatly improved, and the output value has also leapt to the forefront of the world. Tremella fuciformis is the main component in Tremella fuciformis. Studies have shown that Tremella fuciformis polysaccharides are associated with various pharmacological activities such as anti-tumor, anti-oxidation, hypolipidemic, anti-radiation, weight loss, wound healing, enhancement of immunity and prevention of intestinal diseases. . Since the Japanese scholars first extracted polysaccharides from the fungus, the research on the extraction process of Tremella polysaccharides has attracted more and more attention. The extraction methods include solvent extraction (hot water, acid and alkali), enzymatic method, ultrasonic assisted, microwave. Auxiliary extraction, high pressure cooking technology and high voltage pulsed electric field assistance. Among them, the hot water extraction method is the most widely used technology at present, but the research on the optimization of the hot water extraction method of Tremella fuciformis polysaccharides mostly adopts the "orthogonal experimental design method", which makes it limited to the level of influencing factors in the local area. It is difficult to fully reflect the actual situation of the influencing factors. The response surface analysis method directly reflects the influence of the interaction between the factors on the extraction rate of polysaccharides by establishing complex multidimensional spatial surfaces, which can make the experimental results close to reality. With the development of Tremella cultivation and processing technology, there are more and more wastes such as white fungus, broken meat and knots. The waste processing waste is not timely and the amount of waste is more and more. The processing waste of white fungus is not timely or inappropriate. In most cases, it is disposed of directly without treatment, which will seriously pollute the production and living environment and cause great waste of resources. Therefore, how to properly handle and comprehensively utilize the Tremella processing waste needs further development and research. In view of this, this experiment used single factor experiment and Box-Behnken design method to optimize the extraction process of Tremella fuciformis polysaccharides, aiming to establish a quadratic regression model of tremella connective polysaccharide extraction, determine the optimal extraction process, and on this basis the structure and The in vitro antioxidant activity was analyzed and determined to provide theoretical basis and technical support for the correct treatment of Tremella processing waste, in-depth study of functional activity, industrial application and comprehensive utilization. 
