Medium is a manually formulated nutrient for the growth and maintenance of microbial, plant and animal tissues. It usually contains carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water, etc. , pigments, hormones and serum. In order to facilitate long-term storage, the components of the medium are made into a powder. When used, it is formulated into a usable medium, and the entire preparation process medium requires strict sterilization.

At present, the medium is usually sterilized by high-pressure steam, because steam has a strong penetrating ability and can kill various microorganisms. The cause of death of microorganisms is mainly due to high temperature, which causes some important proteins in the microorganisms, such as enzymes. , coagulation, degeneration, resulting in the inability of microorganisms to survive and die. The heating temperature and heating time in sterilization are related to the degree of sterilization and the destruction of nutrients. The destruction of nutrients will affect the culture of the strains and the production of the products. Therefore, the degree of sterilization and the destruction of nutrients have become the main contradiction in the sterilization work. Properly grasping the heating temperature and heating time is the key to the sterilization work. Selecting a temperature and heating time that can meet the sterilization requirements and reduce the destruction of nutrients is an important part of researching the quality of medium sterilization.

From the analysis of the experimental results in the above figure, we can conclude that the temperature of the bacteria increases, the rate of death of the bacteria is greater than the rate of destruction of the medium components. In actual production, in order to achieve the purpose of sterilization, the nutrients can be well preserved. Good use of high temperature rapid sterilization. In order to completely kill the thermophilic spores, we usually use 121 ° C, 15-20 minutes; 135 ° C, 5 minutes. The cooling process after sterilization of the medium is also particularly important. At present, everyone faces a common problem: the medium is cooled too slowly, and the door is cooled and feared of outside air pollution. And the pollution problem of the culture medium in the process of pouring the plate also makes everyone feel a headache, and the reason for the long bacteria in the culture dish has become a question everyone has been asking.

Germany systec invented a special sterilizer - medium preparation device specially prepared for medium sterilization preparation. The advent of this instrument solves many problems that everyone faces:

Cooling: The cooling system has been re-optimized based on systec's advanced sterilizing pot, which greatly reduces cooling time. The mediator is rapidly cooled by circulating water and pressure. The disc-shaped heat exchanger connected to the external water enables rapid internal cooling, including heating, sterilization and cooling (to 50 ° C) depending on the size of the vessel, the amount of medium and the temperature of the cooling water. It takes only 60-120 minutes to complete. The pressure of the container load effectively avoids the over-temperature failure of the medium and the generation of bubbles in the medium.

Safe dispensing problem: The media preparer has a safe media dispensing line and the entire dispensing process is dispensed through a sterile hose. During the sterilization process, the internal absorption tube and distribution tube also require steam sterilization. The external tube and dispensing joint of the instrument should be sterilized using a suitable steam sterilization method and then connected to the dispensing port in the sterilization.

Quality Assurance: Fully automatic control program to ensure uniform internal temperature, thus ensuring a higher quality of the formulated and sterilized medium, the entire process, from initial heating to mixing and sterilization at a set temperature, and finally To cooling, it is microprocessor controlled and the temperature maintained after sterilization is preset. The biocide has a large inlet and a safety lock. The bactericide can be added from this port or can be injected from the silicone film with a syringe.

Automatic dispensing: After the preparation of the medium, it is automatically distributed by the silicone pipe connection and packaging system. The whole packaging system is precisely controlled by the microcomputer host, and the built-in UV lamp is sterilized to ensure the true sterility of the dispensing. For each 20ml reference, the maximum dispensing speed is 800/hour. The entire dispensing process is automatically completed by the preset procedure, no labor cost is required, and a large amount of time cost is saved. The optional cooling function and coding function of the main unit solve the problem of your medium production.

For laboratories with large amounts of culture dishes, let's do a simple cost analysis: If our laboratory needs 1,500 bottles a day, if you buy a finished dish from 5-10 yuan/block from the culture dish manufacturer. At least 7500 per day, if we use Systec's one-button preparation + dispensing system, taking 30L of medium preparation as an example, we can produce 1500 planes for about 4 hours (2 hours of preparation, minutes) Install for 2 hours). The cost of the medium component powder and the cost of the plane were removed: 2 yuan/piece. Every day we can save 4,000 yuan in cost and also guarantee the quality of the medium. Operating by 20 days a month, we can save us about 80,000 in January. The cost of the instrument can be saved in more than half a year. And also has the capacity of the culture dish, which can be used for export. Therefore, many well-known media manufacturers at home and abroad have become our long-term partners: Oxoid, Thermo Fisher, Beijing Luqiao, Antu Green, Shanghai Merière and so on.

If you are not satisfied with the quality of the medium you have prepared, if you are overwhelmed by the medium, if you feel that the time spent on the board is too much, if you are too expensive to buy the dish, you will still be worried. What Systec will be your best choice, you deserve it!