As technology grows, Vehicle Surveillance System is getting maturer and maturer. Vehicle Surveillance System is made of Vehicle Surveillance Cameras, MDVR, Web connecting, and monitor manage system. Now let's get known of the Car Surveillance Camera.
Vehicle Camera has different types according to their installation locations and aims. Generally speaking, a truck/ police car/ tanker truck/ bus should be equipped with several camers not only one, to realize the no blind spot monitoring and keep safe.
Vehicle Surveillance Cameras, Car CCTV Camera, Vehicle Camera, Car Surveillance Camera, Car CCTV, Vehicle CCTV SHENZHEN SANAN TECHNOLOGY CO.,LTD , https://www.sanan-cctv.com
Let's check the following Car CCTV Cameras in details.
Front View Camera: installed on the front of the car, play the role of Driving Recorder;
Driving Position Camera: installed on the driver's position, standardize the driver's behaviors, according to automatic AI analysis. And the system will Automatically remind the driver of his/her bad behaviors and record this event, if the driver gets distracted, smokes, or continues driving under tired status;
In-car Camera: intalled in vehicles, realized the no blind spot monitoring inside the vehicles;
Rear View Camera: installed at the rear of the vehicle, realize the road condition monitoring behind the vehicle;
Therefore, the best way is to customize the suitable Vehicle Surveillance System according to specific application occassions. Sanan Technology has been concentrated on the Vehicle CCTV industry for over 10 years, Vehicle Surveillance System Solution is what we are good at. "Security Anytime Anywhere, Vehicles Surveillance System Specialist" is our slogan, and we are runing for it.
We would be very appreciated if we can get be given the chance to cooperate, and then we would grow together.
Histamine ELISA kit instructions
(Germany IBL: RE59221)
1 , the scope of application
This kit can be used for in vitro quantitative detection of histamine in human plasma and urine. Deepening this experiment can be used for the study of cell culture supernatants.
2 , the preface
Histamine (β-imidazole ethylamine) is the most important medium in the human body, and it is mainly present in the initial stage of allergic reactions (rapid allergy). Histamine is produced by the deamination of histidine. Histamine is present in almost all tissues of the body and is mainly stored in the metachromatic particles of columnar cells and basophils. Histamine is usually present in an inactive complex and will only be released when needed. Like other mediators, histamine not only regulates various clinical symptoms of allergic reactions, but also regulates a range of effects that stop allergic reactions. The biological activity of histamine in tissues is accomplished by three receptors, which are H1, H2 and H3 receptors, respectively. The method for clinical detection of histamine is to quantitatively detect the amount of histamine released from basophilic leukocytes in various allergic allergic reactions, and also to detect various body fluids (plasma, urine, cell culture supernatant) after treatment with allergic drugs. The amount of amine.
3. Experimental principle
This ELISA kit utilizes the principles of competition law. An unknown amount of antigen in the sample and a certain amount of the enzyme-labeled antigen compete for binding to the antibody binding site coated in the microwell. The plate was washed after incubation to terminate the competitive reaction. After the addition of the substrate solution, the color intensity exhibited by the solution is inversely proportional to the amount of antigen in the sample. The experimental results of the sample can be obtained directly from the standard curve.
4 , storage and stability
The kit must be transported and stored at 2-8 ° C to avoid direct sunlight. Sample storage and stability and reagent preparation will be described in the relevant sections. If the coated sheet is sealed and stored in an environment of 2-8 ° C, the reagent is stable for the period of validity.
5 , sample collection and storage
Plasma (EDTA, heparin)
Samples are collected according to routine precautions for venipuncture, and blood samples must be chemically intact from collection to testing. Do not use significant hemolysis, jaundice, and lipemia samples. The turbid sample should be centrifuged before the start of the experiment to remove all particulate matter.
store
2-8 ° C
≤-20°C
≤-70°C
Avoid direct sunlight and avoid repeated freezing and thawing.
stability
5 hours
3 months
2 years
Urine
The experiment must use naturally occurring urine, or use urine generated within 24 hours. The urine produced by the patient within 24 hours was collected and mixed in a container containing 10-15 ml of 6N HCl preservative. To calculate the results, determine the total volume of urine. Centrifuge the sample before starting the experiment.
Natural urine
Acidified urine
Avoid direct sunlight and avoid repeated freezing and thawing.
store
2-8 ° C
2-8 ° C
≤-20°C
stability
8 hours
3 days
6 months
Cell culture supernatant
Use cell culture supernatant as a sample, generally no special considerations
The concentration of histamine in the cell culture medium may be slightly higher.
Whole blood
Heparinized whole blood must be used for the detection of histamine release in whole blood. For specific information, please refer to the corresponding manual (RE95000)
6 , kit components
Note: The kit has enough reagent components for 96 single-well assays or 48 dual-well assays.
Quantity
mark
ingredient
1×12×8
MTP
The coated plate was detachable and the micro-pore was coated with goat anti-rabbit antiserum.
1×5ml
ANTISERUM
Histamine antiserum, blue, ready to use, containing: rabbit antiserum, Tris buffer and 0.01% thimerosal
1×75ul
ENZCONJ CONC
Enzyme conjugate, 200-fold concentrated, containing horseradish peroxidase-labeled histamine.
7×0.4ml
CAL P AG LYO
Plasma standard AG, lyophilized, was used for calibration of plasma samples. Contains: histamine, human plasma. See the reagent bottle label or quality control sheet for specific concentrations.
2 x 0.4 ml
CONTROL P1+2 LYO
Plasma quality control 1 + 2, lyophilized, containing: histamine, human serum. See the reagent bottle label for specific concentrations and allowable ranges.
1 x 2.0 ml
5×0.25ml
CAL U/C AF
Urine/cell culture standard AF, 0; 2.7; 8.1; 24.3; 73; 219 ng/ml, ready for calibration of urine and cell culture samples. Contains: histamine and 0.1 M HCl.
2×0.25ml
CONTROL U/C 1+2
Urine quality control 1 + 2, ready to use, containing: histamine, human urine (acidified). See the reagent bottle label for specific concentrations and allowable ranges.
1×2.25ml
ACYLREAG
Acylation reagent, ready to use, contains: DMF
1×60ml
ASSAYBUF CONC
The assay buffer was concentrated 5 times and contained Tris buffer, Tween, BSA and 0.05% thimerosal.
1×50ml
WASHBUF CONC
The washing solution was concentrated 20 times and contained phosphate buffer, Tween and 0.1% thimerosal.
1×10ml
INDICATORBUF
Indication buffer, purple, ready to use, contains: Tris buffer, phenol (color changes when pH < 7.5 = and 0.1% thiomersal.
1×12ml
TMB SUBS
TMB substrate solution, ready to use, contains TMB, buffer and stabilizer.
1×12ml
TMB STOP
TMB stop solution, ready to use, 1 M H 2 SO 4 .
3×
FOIL
Sticky metal plate
7. Equipment required for the experiment but the kit does not provide
1) Pipette, volume: 10; 20; 50; 100; 1000ul
2) Test tube (12 × 75mm)
3) Test tube rack
4) Oscillator
5) Vortex mixer (500 rpm)
6) 8-channel pipette with reservoir
7) Washing bottles, automatic or semi-automatic washing machine
8) Microplate reader (reference wavelength: 600-650nm)
9) distilled or deionized water
10) Absorbent paper, sampling tips and timer
8. Preparation instructions before the experiment
note
The 96-person kit can be tested in three separate portions, and the volume described below is the volume required to detect 32-person portions.
8.1 Preparation of lyophilized or concentrated ingredients
Dilution/dissolution
ingredient
Thinner
proportion
Remarks
store
stability
20ml
Detection buffer
100ml
Distilled water
1:5
2-8 ° C
2 weeks
15ml
detergent
300ml
Distilled water
1:20
Dissolve the crystal at 18-25 ° C
2-8 ° C
4 weeks
Plasma standard
0.4ml
Distilled water
Allow to stand for 15 min, no foam when mixing
-20 ° C
1 month
Plasma control
0.4ml
Distilled water
Allow to stand for 15 min, no foam when mixing
-20 ° C
1 month
10ul(*)
Enzyme complex
2ml
Slow detection
Flush
1:200
Temporary configuration, can only be used once
18-25
°C
30 minutes
(*) Make sure there is no residual liquid in the plug before diluting.
8.2 dilution of the sample
Samples with higher histamine concentrations in the sample than the highest standards should be diluted with the appropriate dilutions prior to acylation. 0.1M HCl for urine samples and sample dilutions for plasma samples (Cat.no. KEHP711), kit Not available inside.
8.3 Acylation of the sample
Prepare samples with test tubes
note
The plasma standard curve cannot determine the concentration of histamine in the acylated urine or cell culture sample, nor can the U/C standard curve be used to determine the concentration of histamine in the acylated plasma sample.
8.3.1 Plasma
1
Plasma standards, plasma controls, and patient samples were separately added to the corresponding tubes in 100 ul.
2
100 ul of indicator buffer was added to each tube and vortexed.
3
20 ul of the acylating reagent was added to each tube, and the acylating reagent was added and vortexed immediately.
4
The tube was capped and incubated for 30 minutes at room temperature (18-25 ° C).
5
750 ul of the diluted assay buffer was added to each tube and vortexed.
8.3.2 Urine, cell culture supernatant
1
Add 50 ul of U/C standard, urine control, and patient urine or cell culture supernatant to the corresponding tubes.
2
50 ul of indicator buffer was added to each tube and vortexed. If the indicator becomes colorless, the pH of the solution is very low and the sample contains a lot of acid. In this case, 50 ul of indicator buffer was added to the tube until the solution was slightly reddish.
3
10 ul of the acylating reagent was added to each tube, and the acylating reagent was added and vortexed immediately.
4
The tube was capped and incubated for 30 minutes at room temperature (18-25 ° C).
5
2000 ul of diluted assay buffer was added to each tube and vortexed.
Note: The acylated sample can be stored at 2-8 ° C for one night and stored at -20 ° C for 2 days.
9 , experimental steps
1
50 ul of the acylated standard, control, and patient samples were added to the corresponding microwells, respectively.
2
50 ul of temporarily configured enzyme conjugate was added to each well.
3
50 ul of histamine antiserum was added to each well.
4
The cover plate, shaker (500 rpm) was incubated for 3 hours at room temperature.
5
The viscous metal plate was removed, the contents of the wells were discarded, and the plate was washed 4 times with 250 ul of diluted washing solution per well, and the absorbent paper was patted dry to remove residual liquid.
6
If available, an 8-channel pipette can be used to add substrate and stop solution. When adding the substrate solution and the stop solution, the time interval of each well should be the same. Use an active displacement pipette and avoid air bubbles.
7
100 ul of TMB substrate solution was added to each well.
8
Plasma: Oscillator (500 rpm) was incubated for 40 min at room temperature.
Urine/cell culture supernatant: Oscillator (500 rpm) was incubated for 20 min at room temperature.
9
100 ul of TMB stop solution was added to each well to terminate the substrate reaction. Gently shake the plate to mix the solution evenly.
10
The OD value was read at 450 nm within 15 min after the addition of the stop solution. (Reference wavelength: 600-650nm)
10 , expected value
The results of the experiment cannot be considered as the sole cause of any treatment outcome, and the judgment of the disease should be combined with other clinical observations and diagnostic tests. The following results are normal values ​​(5%-95%) for the normal population. It is recommended that each laboratory read and determine its normal range of values:
plasma
Urine
24-hour urine
Naturally produced urine
Ng/ml
Ug/d
Ug/g creatinine
0.2-1.0
5-56
8-53
This translation is for reference only, please refer to the original for details.
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