This kit can only be used for scientific research and should not be used for medical diagnosis. Porcine Complement Protein 3 (C3) ELISA Kit Instructions for Use Detection principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells of the pre-coated complement protein 3 (C3) antibody, the specimen, the standard, and the HRP-labeled detection antibody were sequentially added, and the cells were washed and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with complement protein 3 (C3) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a tube containing no pyrogen and endotoxin. Avoid any cell irritation during the procedure. After collecting the blood, centrifuge and centrifuge for 10 minutes at 3000 rpm to quickly and carefully separate the serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. The supernatant was taken by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymer. 4. Tissue homogenization: The tissue is mashed by adding appropriate amount of physiological saline. The supernatant was taken by centrifugation at 3000 rpm for 10 minutes. 5. Storage: If the sample is not detected in time after collection, please dispense it once, freeze it at -20 °C, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is fully thawed evenly. Bring your own items Operational precautions Kit composition name 96-well configuration 48 hole configuration Remarks Microporous ELISA plate 12 holes × 8 12 holes × 4 no Standard 0.3mL*6 tube 0.3mL*6 tube no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20× washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instruction manual 1 copy 1 copy no Ziplock bag 1 1 no Note: The concentration of standard (S0-S5) is: 0, 5, 10, 20, 40, 80μg/mL Reagent preparation Dilution of 20× Wash Buffer: Distilled water was diluted 1:20, ie 1 part of 20× Wash Buffer plus 19 parts of distilled water. Washing method Steps Result judgment Draw a standard curve: In the Excel worksheet, the standard concentration is used as the abscissa, and the OD value is plotted as the ordinate. The linear regression curve of the standard is drawn, and the concentration values ​​of each sample are calculated according to the curve equation. Kit performance Disclaimer Shanghai Yuduo Biotechnology Co., Ltd. produces and sells elisa kit, animal serum, plasma, whole blood, antigen and antibody, gold standard kit test card, antiserum, biological reagents, culture medium, laboratory equipment consumables, chemical reagents, biological products. , standard products, reference products, biochemical immunological products, immunoaffinity columns, strains, plasmids, indoor quality control products, cells, ice packs, agent imported products, etc. Large favorably! Welcome dealers to discuss cooperation matters! Contact Phone Contact QQ
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A laser hair growth cap, also known as a laser hair therapy cap or laser hair regrowth cap, is a wearable device that uses low-level laser therapy (LLLT) to stimulate hair growth. It is designed to treat hair loss and promote hair regrowth in individuals experiencing hair thinning or balding.
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The cap contains multiple laser diodes or LEDs that emit red or near-infrared light. When the cap is worn on the head, the lasers or LEDs penetrate the scalp and stimulate the hair follicles. This stimulation promotes blood circulation, increases cell metabolism, and triggers hair growth.
Laser hair growth caps are typically used for a specified duration per session, usually around 20-30 minutes, a few times per week. The treatment duration can vary depending on the individual's needs and the specific cap model.
It is important to note that laser hair growth caps are not a guaranteed solution for hair loss, and results may vary from person to person. They are often used in conjunction with other hair loss treatments, such as medications or topical solutions, for more comprehensive results.